|Portrayed by:||Jennifer Connelly|
|First appeared:||Spider-Man: Homecoming|
|Appears in:||1 Film|
Karen is a natural-language user interface created by Tony Stark and installed into Spider-Man Suit, designed to work alongside and aid Spider-Man in his crime-fighting activities, provided after completing the Training Wheels Protocol.
After Peter Parker discovered that his Spider-Man Suit was being restricted by the Training Wheels Protocol, he had his friend Ned Leeds disable it. Deciding to prove his worth to Tony Stark by locating the source of an illegal arms deal, the Karen A.I. was activated, much to his surprise. There, Karen congratulated him for completing his training and alerted him that he had many different web shooter combinations available. Still working out the improved suit, Parker was exposed to several different web styles in the heat of combat. Shortly after trying to intervene in Vulture's theft, Parker was ambushed by the Vulture himself. After a brief fight atop the moving vehicle, Vulture overpowered Parker and left him unconsciousness in the truck.
Parker later awoke in the Damage Control Deep Storage Vault in Washington, D.C.. Karen informed Parker that the only way out was through the sealed bay doors, which would not open until morning. With seemingly nothing to do but wait out the night, Parker discussed his suit's capabilities with his A.I, naming her "Karen".
During the night Parker was trapped in the Damage Control safehouse, it came to Karen's attention that a power core Parker happened to be holding was a dangerous explosive that detonates when exposed to enough radiation. Remembering that Ned Leeds had another lethal power core with him, Peter Parker immediately looked for ways out. Karen informed Peter that the bay doors could be opened if its security was overridden, but it would take most of the night to try every possible route. Roughly 200 trials into hacking the doors open, morning had come and Parker managed to open the doors. He requested Karen find the fastest possible route to Washington DC, where Leeds was.
Following Aaron Davis' lead, Spider-Man sneaked into the ferry and went looking for Vulture. He learned that they were about to deal with a criminal named Mac Gargan to which Karen provided information about.
While Tony Stark was calling and speaking to Spider-Man however, he could see that Randy Vale was preparing to hand over the Chitauri weapons to Gargan and the other criminals, so Spider-Man was forced to hang up on the annoyed Stark in order to focus on his targets before it was too late and Gargan got away. Spider-Man interrupted the deal and began fighting the crew and Gargan. Spider-Man fought against Herman Schultz who has gained possession of the Shocker's Gauntlet from Jackson Brice and then attempted to strike him with it. Spider-Man managed to defeat them, knocking Gargan overboard and trapping Schultz's arm but was then interrupted by FBI who began arresting everyone including Spider-Man who they mistook for Gargan's ally, much to his surprise as he tried to insist that he was not the criminal in this situation.
This distraction was all the Vulture needed to don his suit and began attacking Spider-Man and the FBI. While Spider-Man protected and saved the agents from the villain's attacks, Vulture freed his men and urged them to escape. Another fierce fight ensued as Vulture tried to kill Spider-Man with his advanced weaponry. Spider-Man countered by using his Web-Shooters advanced functions to even the odds, webbing Vulture to a car in an attempt to slow him down.
Vulture managed to free himself of Spider-Man's webs by cutting through them with his wings and continued attacking him. Eventually, the fight resulted in Vulture's weapon malfunctioning. Spider-Man tried to webbed the weapon up only for Vulture to say he was dealing with things he did not understand. The weapon then exploded and cut the ferry in half. Vulture escaped as Spider-Man desperately tried to save the people inside the ferry.
Karen quickly calculated the strongest points of the ship and Spider-Man quickly went to work by webbing the strongest points together. Using his web-shooter and his own enhanced speed, Spider-Man seemingly managed to hold the ship together only to learn that his missed one spot and the ship starts collapsing. In desperation, Spider-Man webbed and painfully held both halves of the ship by himself in a herculean effort to save the ship.
Spider-Man noticed that the ship wasn't sinking anymore and it stopped being painful to hold the ship together. Iron Man revealed himself and has come to rescue the civilians. He quickly rebuilds the ship by reforging the cutlines.
Karen was jokingly referred to by director Jon Watts as "J.A.R.V.I.S.' wife".